A dot-blot assay for the low density lipoprotein receptor.
نویسندگان
چکیده
منابع مشابه
Familial Hypercholesterolemia in Iran: A Novel Frameshift Mutation in Low Density Lipoprotein Receptor (LDLR) Gene
Background and Objective: Familial hypercholesterolemia (FH) is an autosomal trait, which is caused by mutations in Low Density Lipoprotein Receptor (LDLR) gene. FH penetrance is about 100% and worldwide prevalence for heterozygous subjects is almost 1 in 500 and for homozygous 1 in 1,000,000. The patients are at risk of premature coronary heart disease (CHD) due to defective LDLR a...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملA Novel Mutation in Exon 4 of the Low Density Lipoprotein (LDL) Receptor Gene in an Iranian Familial Hypercholesterolemia Patient
Familial hypercholesterolemia (FH) is an autosomal co-dominant disorder of lipid metabolism, caused by mutations in LDL receptor gene. The penetrance of FH is almost 100%, meaning that half of the offspring of affected parents born with disease. The patients are at risk of premature coronary heart disease (CHD). There is no report about the molecular basis of FH in Iran. Identification of mutat...
متن کاملRole of the Low Density Lipoprotein Receptor
A B S TRA CT The transfer of normal human fibroblasts from medium containing whole serum to medium devoid of lipoproteins produced a 90% decrease in the cellular content of cholesteryl esters and a 30% decrease in the free cholesterol content. When these lipoproteindeprived cells were subsequently incubated with human low density lipoprotein (LDL), there was a 7-fold increase in the cellular co...
متن کاملA red-dot-blot protein assay technique in the low nanogram range.
A simple, sensitive, rapid (3 min), and highly reproducible solid-phase assay for the detection of proteins in the low nanogram range (4 ng) is described. The assay is based on differential Ponceau S staining of the protein spots on nitrocellulose and quantification of the protein-dye complexes on lubricated membranes using a densitometer. The dye solution used for protein staining contained 0....
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1986
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38728-9